Protein microarray technology has transformed the fields of genomics and proteomics. This groundbreaking technology uses assays to immobilize a large sample of proteins for detection and study. This useful tool has experienced exponential growth in many biological fields; protein microarrays are the driving force behind advancements in genetics and disease diagnosis. These microarrays are a cost effective option that scientists can leverage to understand protein interaction, development, and modification. A proteome array is an extremely valuable biological tool; here is the process involved with performing a protein microarray.
Protein Microarray: Design and Assembly
There are three key ingredients in a protein microarray: a microarray printer, antigens of interest, and a surface to print the proteins on. All of this required equipment can be designed independently or purchased from a commercial vendor. Once these pieces are obtained and assembled, a sample of antigens are loaded into the wells on a microarray printer; the average microarray printer can print 100 microarrays in one day.
Probing and Detection
After the microarrays are printed on a surface, this medium is then soaked in a blocking buffer to minimize non-specific binding. After the microarrays are probed, a secondary antibody is applied and the concentration is incubated. Following incubation, microarrays are washed and dried.
Image Processing and Data Analysis
One microarrays are dried they are ready for analysis. You can obtain high-resolution microarray images through using a microarray image processing software. The layouts of antigens that appear on the array are stored in files, which can be uploaded into the imaging software. Once the images are stored in flies on the computer, they are ready to be used for analysis.
Protein microarrays are a valuable tool for understanding the complex proteins coded in your genome. This advanced technology helps scientists understand the ways proteins in your body are functioning in cellular environments.